bioimaging and optics platform BIOP

MASTER PROJECTS 2016

 

Project A -2016

Mechanisms Of Centriole Assembly Analyzed By Fluorescence Correlation Spectroscopy (FCS)

Investigating protein-protein interactions in living organisms is key for understanding fundamental processes such as cell division. However, classical light microscopy is diffraction-limited and, therefore, inappropriate in terms of resolution, especially for imaging small cellular organelles. Fluorescence Correlation spectroscopy (FCS) is an elegant approach to overcome this limitation by measuring intensity fluctuations of a fluorescently labeled molecule (e.g. a protein tagged with GFP).
In this master project, you will use FCS to analyze the behavior of SAS-5 and SAS-6, two proteins that are essential for centriole assembly in the nematode C. elegans. The centriole is a small organelle critical for the formation of cilia and flagella and involved in a variety of important biological processes. 
The work will be conducted under the joint supervision of Nicola Brown, an experienced post-doctoral fellow in the laboratory, and Arne Seitz, the head of the BioImaging and Optics core facility of the School of Life Sciences. Contact Prof. Pierre Gönczy if you are keen on learning and applying this state of the art light-microscopy method in the context of this exciting project! 
Recommended for STI and SV students.

 

Project B -2016

Photoconversion and photoactivation of fluorescent proteins and their implications on FRET meaurements

The couple of CFP/YFP is commonly used in FRET microscopy and although other combinations of fluorescent proteins have been reported to be advantageous it is still widely used. However this combination suffers from a variety of limitations. Recently, wavelength dependent photoactivation and photoconversion phenomena have been reported which especially compromise intensity based FRET measurements. Fluorescence Lifetime microscopy(FLIM) is a powerful approach in order to make FRET measurements more reliable and turn them even into a quantitative tool.
In this master project, you will use FLIM in order to develop a workflow allowing the reliable measurement of FRET efficiencies. This will allow to measure the distance between two interacting proteins on the nanometer scale.
The work will be conducted under the joint supervision of Romain Guiet, an experienced post-doctoral fellow in the laboratory, and Arne Seitz, the head of the BioImaging and Optics Core Facility of the School of Life Sciences. Contact Arne Seitz if you are keen on learning and applying this state of the art light-microscopy method in the context of this exciting project!
Recommended for STI and SV students.

Project C -2016

Validation of Image analysis pipeline for drosophila wing disk development

Growth and patterning occur concurrently in the developing organs of all multi-cellular organisms from flies to humans. Their coordination is of paramount importance to yield functional organs of proper size and shape. Microscopy and image processing are versatile tools to careful quantify growth parameters.
This master project, aims to validate an already existing image processing workflow for the tracking of nuclei throughout drosophila wing disk development. Nuclei are visualized with a lineage tracing method called "Raeppli". It permanently marks a cell with one of 4 different fluorescent markers at the desired time point. Importantly, all progeny of the first cell inherits the color-code forming a "cluster".
The work will be conducted under the joint supervision of Olivier Burri, an experienced image analysist in the laboratory, and Arne Seitz, the head of the BioImaging and Optics core facility of the School of Life Sciences and in collaboration with Fisun Hamaratoglu (UNIL) . ContactcArne Seitz if you are keen on learning and applying this state of the art image processing method in the context of this exciting project!
Recommended for STI and SV students.



Contact

BioImaging and Optics platform (PTBIOP)

EPFL-SV-PTECH-BIOP
Station 15
CH-1015 Lausanne
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Dr. Arne Seitz

Office AI-0240
Tel: +41 (0) 21 693 96 18
Fax: +41 (0) 21 693 95 85