- English ONLY
bioimaging and optics platform BIOP
It requires a stack of 2D images (usually a time-series) and a line selection (= region of interest; ROI). The Kymograph (or time space-plot) is generated by copying the pixel-values of the selected ROI into a new image. This is done consecutively for all frames of the input stack.
The collection also contains methods that allow calculating various kymographs of extended lines. This width of the line is defined by a parameter the user is requested to prompt after the plugin was called.
Three different methods exist in order to "shrink" the extended line (Sum, Min, May).
In order to install the collection of plugins the "Kymo_graph1001.jar" file just needs to be copied into the "plugins" folder of ImageJ.
The Kymograph Plugin under the Fiji Plugins Menu
Notes: The ROIs must be either lines or segmented lines, otherwise the plugin will exit with a warning.
Processive movement of single kinesins on crowded microtubules visualized using quantum dots. Seitz A, Surrey T., EMBO J. 2006 Jan 25;25(2):267-77. Epub 2006 Jan 12. PMID: 16407972