bioimaging and optics platform BIOP

SPLASH

LEICA SP5 MULTI-PHOTON


SPECIFICATIONS:

OBJECTIVES
Objective Magnif. / NA Medium Contrast Working Distance
HC PL APO 10x/0.40 IMM - 0.36mm
HCX PL APO 63x/1.20 water corr 0.22mm
HCX APO* 20x/1.00 water - 2.00mm






LASERLINES AND(COHERENT laser adjustable from 690nm to 1080nm)
FIXE Wavelengths Avaibility Laser type and power Some dyes and use
633nm YES HeNe laser, --mW DRAQ5, CY5, Alexa648



FILTERCUBES for Mercury lamp
Fluorochromes Avaibility Excitation Dichroïc Emission Filter No
Blue YES BP 344-380 FT 400 LP 425 A
Green YES BP 450-490 FT 510 LP 515 I3
Red YES BP 515-560 FT 580 LP 590 N2.1

FILTERCUBES for LASER => Fluorescence
Fluorochromes Avaibility Signal BS BArrier ch1 Barrier ch2 Order Nr.
FITC / TRITC YES RSP 560 ET 525/50 HC 585/40 15 6504
DAPI / FITC YES RSP 495 HC 457/50 ET 525/50 15 6504 241
DAPI / TRITC YES RSP 495 HC 457/50 HC 585/40 15 6504 242
CFP / YFP YES RSP 505 HC 483/32 ET 535/30 15 6504 243
FITC / TXR YES RSP 560 ET 525/50 ET 617/73 15 6504 244

FILTERCUBES for LASER => SECOND HARMONIC
Compagny Avaibility filter Lot Number
Semrock YES FF01-680/SP 209350-209353
Semrock YES FF01-474/10 608337-508346
Semrock YES FF01-445/20 406367-506338
Semrock YES FF01-525/50 109336


Contact

BioImaging and Optics platform (PTBIOP)

EPFL-SV-PTECH-BIOP
Station 15
CH-1015 Lausanne
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Dr. Arne Seitz

Office AI-0240
Tel: +41 (0) 21 693 96 18
Fax: +41 (0) 21 693 95 85