bioimaging and optics platform BIOP

SP8 Acquisition

LEICA SP8 FLIM


SPECIFICATIONS:

Leica SP8 With FLIM
LOCATION AI-0132
(main entrance AI-0133)
Direct reservation for Leica SP8

Person in charge:
Luigi Bozzo
OBJECTIVES
Objective Magnif. / NA Medium Contrast Working Distance
HCX PL Fluotar 10x/0.30 air - 11.0mm
HC FLUOTAR 25x/0.95 Water - 2.40mm
HC PL APO 63x/1.20** water + corr - 0.30mm
HC PL APO 63x/1.30 glycerol + corr - 0.30mm
HC PL APO 63x/1.40 oil + corr - 0.14
**Motorized correction ring and microfluid dispenser






LASERLINES
FIXED Wavelengths Avaibility Laser type and power Some dyes and use
405nm YES Diode laser DAPI, Hoechst, PA, Uncaging
440nm YES Diode laser CYanFP

VARIABLE White light laser: Tunable from 470nm to 670nm in 1nm increments.




FILTERCUBES for Mercury lamp
Fluorochromes Avaibility Excitation Dichroïc Emission Filter No
Blue YES BP 344-380 FT 400 LP 425 A
Green YES BP 450-490 FT 510 LP 515 I3
Red YES BP 515-560 FT 580 LP 590 N2.1
Blue/Green YES


Contact

BioImaging and Optics platform (PTBIOP)

EPFL-SV-PTECH-BIOP
Station 15
CH-1015 Lausanne
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Dr. Arne Seitz

Office AI-0240
Tel: +41 (0) 21 693 96 18
Fax: +41 (0) 21 693 95 85