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Kymograph Plugin

Dr. A. Seitz.

This collection of plugins is aiming to facilitate the measurement of velocities.


It requires a stack of 2D images (usually a time-series) and a line selection (= region of interest; ROI). The Kymograph (or time space-plot) is generated by copying the pixel-values of the selected ROI into a new image. This is done consecutively for all frames of the input stack.

The collection also contains methods that allow calculating various kymographs of extended lines. This width of the line is defined by a parameter the user is requested to prompt after the plugin was called.

Three different methods exist in order to "shrink" the extended line (Sum, Min, May).

In order to install the collection of plugins the "Kymo_graph1001.jar" file just needs to be copied into the "plugins" folder of ImageJ.

Some Image

The Kymograph Plugin under the Fiji Plugins Menu


Download Kymnograph Plugin

Notes: The ROIs must be either lines or segmented lines, otherwise the plugin will exit with a warning.


Processive movement of single kinesins on crowded microtubules visualized using quantum dots. Seitz A, Surrey T., EMBO J. 2006 Jan 25;25(2):267-77. Epub 2006 Jan 12. PMID: 16407972


BioImaging and Optics platform (PTBIOP)

Station 15
CH-1015 Lausanne
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Dr. Arne Seitz

Office AI-0240
Tel: +41 (0) 21 693 96 18
Fax: +41 (0) 21 693 95 85