bioimaging and optics platform BIOP

SPLASH

NIKON SIM and STORM


SPECIFICATIONS:

OBJECTIVES
Objective Magnif. / NA Medium Contrast Working Distance
PLAN APO 10x/0.45 air - 4.0mm
PLAN APO λ 20x/0.75 air - 1.0mm
PLAN APO IR 60x/1.27 Water - 0.16 to 0.18mm
APO TIRF 100x/1.49 oil - 0.12mm






LASERLINES STORM
FIXE Wavelengths Avaibility Laser type and power Some dyes and use
402nm STORM Coherent Cube 100mW
DAPI, Hoechst, PA, Uncaging
488nm STORM Coherent Saphire 480mW
GFP, Alexa488, CY2
561nm STORM Coherent Saphire 480mW
TexasRed, Alexa561, CY3
640nm STORM  
DRAQ5 (nuclear staining), CY5, Alexa648
LASERLINES SIM
FIXE Wavelengths Avaibility Laser type and power Some dyes and use
402nm SIM Coherent Cube 100mW
DAPI, Hoechst, PA, Uncaging
488nm SIM Coherent Saphire 480mW
GFP, Alexa488, CY2
561nm SIM Coherent Jive 158mW
TexasRed, Alexa561, CY3
641nm SIM Coherent Cube 100mW
DRAQ5 (nuclear staining), CY5, Alexa648



FILTERCUBES STORM
Fluorochromes Avaibility Excitation Dichroïc Emission Filter No
EGFP YES 472/30 495 520/35 AHF 36-525
TxRed YES 562/40 593 624/40 AHF 36-504
CY 5 YES 628/40 660 692/40 AHF 36-523
N-STORM YES   DM 660    
Quad Band STORM YES 390 482 563 640 405 488 561 640 446 523 600 677 AHF
FILTERCUBES SIM
Fluorochromes Avaibility Excitation Dichroïc Emission Filter No
DAPI YES        
GFP YES        
TxRED YES        
CY5 YES        
FILTERCUBES ON DEMAND
Fluorochromes Avaibility Excitation Dichroïc Emission Filter No
DAPI on demand 377/50 409 447/60 AHF 36-500
  on demand   643 667/30 AHF 48-643 and AHF 47-667
  on demand   643 732/68 AHF 48-643 and AHF 39-732
HALF MIRROR on demand        


Contact

BioImaging and Optics platform (PTBIOP)

EPFL-SV-PTECH-BIOP
Station 15
CH-1015 Lausanne
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Dr. Arne Seitz

Office AI-0240
Tel: +41 (0) 21 693 96 18
Fax: +41 (0) 21 693 95 85